//Video file that you wish to calculate Delta F/Fo on should be open in Fiji
run("32-bit");
x = getTitle();
Stack.getDimensions(width, height, channels, slices, frames);
rename("original");
//Change the number in the line below to reflect the number of frames you wish to use as Fo (baseline)
run("Z Project...", "stop=30 projection=[Average Intensity]");
selectWindow("original");
imageCalculator("Subtract create stack", "original","AVG_original");
close("original");
selectWindow("Result of original");
imageCalculator("Divide create stack", "Result of original","AVG_original");
close("AVG_original");
close("Result of original");
selectWindow("Result of Result of original");